Abstract: OBJECTIVE To establish the method of characteristic spectrum analysis on Dendrobium officinale of Fengdous by HPLC. METHODS HPLC was used with the Zorbax SB C₁₈ column, the acetonitrile-0.2%methanoic acid solution with gradient elution was employed as a mobile phase, detection wavelength was 270 nm, column temperature was 35 ℃, and flow rate was 1.0 mL·min^-1. RESULTS Thirty six common peaks were separated in Dendrobium officinale of Fengdous, the similarities of 10 batches were 0.898-0.969. CONCLUSION The method is accurate and reliable, has a better reproducibility, and provides a reference for the quality control of Dendrobium officinale of Fengdous.