HPLC测定红曲黄酮片中总蒽醌的含量

Determination of Anthraquinone in Hongquhuangtong Pill by HPLC

摘要: 目的建立高效液相色谱法测定红曲黄酮片中总蒽醌的含量。方法以Waters Sunfire C18(250 mm×4.6 mm，5 μm)为色谱柱，甲醇-乙腈-0.1%磷酸(25∶40∶35)为流动相，流速1.0 mL·min^-1，柱温35 ℃，检测波长254 nm。结果色谱峰分离度良好，大黄素在11.50~115.04 ng、大黄酚在12.24~122.40 ng、大黄素甲醚在4.87~48.71 ng内呈良好线性关系。平均加样回收率大黄素为100.6%，大黄酚为101.4%，大黄素甲醚为97.7%。结论该方法简单、快速、重复性好，可用于红曲黄酮片中总蒽醌的质量控制。

Abstract: OBJECTIVE To establish a method for determining the content of anthraquinone in Hongquhuangtong pill by HPLC. METHODS Waters sunfire C18 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase was consisted of methanol, acetonitrile and 0.1% phosphoric acid (25∶40∶35). The flow rate was 1.0 mL·min^-1. The column temperature was set at 35 ℃. The detector wavelength was 254 nm. RESULTS Emodin, chrysophanol, and physcion showed a good linear relationship with range in 11.50-115.04 ng, 12.24-122.40 ng, and 4.87-48.71 ng, respectively; and the recovery were 100.6%, 101.4%, and 97.7%. CONCLUSION The method is simple, rapid, accurate and reliable. And it can be applied to control the quality of anthraquinone from Hongquhuangtong pill.