HPLC测定氟康唑莫西沙星眼用即型凝胶的含量

Determination of Fluconazole and Moxifloxacin in Situ Forming of Eye Gel by HPLC

摘要: 目的建立高效液相色谱法测定氟康唑莫西沙星眼用即型凝胶含量，同时测定制剂中氟康唑、莫西沙星和羟苯乙酯的含量。方法采用ODS-C₁₈色谱柱，以三乙胺缓冲液-甲醇为流动相，流速1.0 mL·min^-1，检测波长260 nm。结果氟康唑、莫西沙星、羟苯乙酯的线性范围分别为25~250 mg·mL^-1(r=0.999 9，n=6)，25~250 mg·mL^-1(r=0.999 9，n=6)，2.5~25 mg·mL^-1(r=0.999 7，n=6)，平均回收率分别为99.6%，99.4%，99.3%。RSD分别为1.23%，1.20%，1.17%。结论本方法操作简便，灵敏度高，重复性好，可用于该制剂的含量测定。

Abstract: OBJECTIVE To establish an HPLC method for measuring the content of fluconazole, moxifloxacin and ethylparaben in situ forming of eye gel. METHODS ODS-C₁₈ column was used, triethylamine buffer-methanol as mobile phase, the flow rate was 1.0 mL·min^-1, detection wavelength was 260 nm. RESULTS The linear range of fluconazole, moxifloxacin and ethylparaben were 25-250 μg·mL^-1(r=0.999 9, n=6), 25-250 μg·mL^-1(r=0.999 9, n=6), 2.5-25 μg·mL^-1 (r=0.999 7, n=6), the average recovery rates were 99.6%, 99.4%, 99.3%. The RSD were 1.23%, 1.20%, 1.17%. CONCLUSION The method is simple, accurate, sensitive and the reproductivity is good. It can be used for the determination of this preparation.