Abstract: OBJECTIVE To develop an HPLC method for determination of six indicative components(baicalin, wogonoside, baicalein, wogonin, chrysin, oroxylin-A) in Scutellaria baicalensis Georgi. METHODS Agilent ZORBAX SB-C₁₈(250 mm×4.6 mm, 5 μm)was adopted; the mobile phase was acetonitrile(A)-0.3% phosphoric acid-triethylamine (B) with gradient elution, and the detection wavelength was set at 275 nm, and flow rate was 1. 0 mL·min^-1 with the column temperature of 30 ℃. RESULTS The content of six elements in Scutellaria baicalensis Georgi was stable. The method had a good linearity in the ranges of 0.416 7-4.167 μg(r=0.999 2) for baicalin, 0.078 15-0.781 5 μg(r=0.999 5) for wogonoside, 0.053 21-0.532 1 μg(r=0.999 3) for baicalein, 0.016 36-0.163 6 μg(r=0.999 3) for wogonin, 0.001 160-0.011 60 μg(r=0.999 1) for chrysin, 0.010 46-0.104 6 μg(r=0.999 4) for oroxylin-A. The average recoveris(n=5) were 99.4%,100.1%, 99.9%, 100.2%, 100.4%, 100.1%, respectively with RSDs of the recoveries were 1.9%, 2.2%, 2.3%, 1.6%, 2.2%, 2.1%, respectively. CONCLUSION This method is acurate, simple and practical,and may be use to control quality of Scutellaria baicalensis Georgi.