Abstract: OBJECTIVE To prepare an appropriate diammonium glycyrrhizinate phospholipid complex injection, and examine its initial stability. METHODS The influence of different solvents and pH values were investigated, using particle size, peroxide value and pH as indicators. The optimal formulation was determined by factor experiment, and undergone acceleration experiment. RESULTS The physical and chemical properties of 5% gluconse formulation did not change significantly under 10 days of high temperature and illumination, as well as under acceleration experiment. CONCLUTION The diammonium glycyrrhizinate phospholipid complex 5% gluconse injection was stable. It meets the requirements of liver targeting.