HPLC测定片剂中阿戈美拉汀的含量

    Determination of Agomelatine in Tablets by HPLC

    • 摘要: 目的 采用HPLC测定片剂中阿戈美拉汀的含量。方法 色谱柱为Phenomenex Luna-C18(250 mm×4.6 mm,5 μm),流动相为甲醇-0.05 mol·L-1磷酸二氢钠溶液(40∶60),流速为1.0 mL·min-1,检测波长为230 nm, 柱温为25 ℃。结果 线性回归方程为Y=1.979×105X-1.258×104(r=0.999 9,n=5),线性范围为1.07~10.7 μg·mL-1,平均回收率为98.73% (n=9),RSD为1.28%。结论 所建方法简便、准确、专属,可用于片剂中阿戈美拉汀的含量测定。

       

      Abstract: OBJECTIVE To establish a method for the content determination of Agomelatine tablets by HPLC. METHODS The HPLC method was performed on Phenomenex Luna-C18 column (250 mm×4.6 mm, 5 μm) with mobile phase of methanol-0.05 mol·L-1 sodium dihydrogen phosphate (40∶60). Flow rate was 1.0 mL·min-1. Detective wavelength was set at 230 nm and the column temperature was set at 25 ℃. RESULTS The calibration curve of Agomelatine was linear Y=1.979×105X-1.258×104 (r=0.999 9, n=5) in the range of 1.07-10.7 μg·mL-1. The average recovery of Agomelatine was 98.73% (n=9) with RSD of 1.28%. CONCLUSION The method is simple, accurate, specific and it can be used for the determination of the content of Agomelatine tablets.

       

    /

    返回文章
    返回