不同产地野菊花中绿原酸、4, 5-二咖啡酰奎尼酸的含量测定

Content Determination of Chlorogenic Acid and 4,5-O-Dicaffeoylquinic Acid in Chrysanthemi Indici Flos from Different Areas

摘要: 目的建立不同产地野菊花中绿原酸、4,5-二咖啡酰奎尼酸的含量测定方法。方法采用HPLC，色谱柱为Diamonsil C₁₈(4.6 mm×250 mm，5 μm)；采用梯度洗脱；检测波长327 nm；柱温30 ℃；流速1.0 mL·min^-1。结果绿原酸、4,5-二咖啡酰奎尼酸的线性范围分别为0.011~0.22 μg，0.017 5~0.35 μg。加样回收率分别为99.1%和98.8%；RSD分别为1.93%和1.54%(n=9)。结论本方法操作简便，分析速度快，结果准确，重复性好，可用于野菊花中绿原酸和4,5-二咖啡酰奎尼酸含量的测定。

Abstract: OBJECTIVE To determine the content of chlorogenic acid and 4,5-O-dicaffeoylquinic acid in Chrysanthemi Indici Flos from different areas. METHODS HPLC was used. The chromatographic separation was performed on a Diamonsil C₁₈(4.6 mm × 250 mm, 5 μm) column with gradient elute. The detection wavelength was 327 nm and column temperature was 30 ℃. RESULTS Good linear relationship between the peak area and content of chlorogenic acid and 4,5-O-dicaffeoylquinic acid was achieved when the amount was within 0.011-0.22 μg, 0.0175-0.35 μg; the recoveries were 99.1% and 98.8%; RSD were 1.93% and 1.54%(n=9). CONCLUSION The method is accurate, reproducible and suitable to determine chlorogenic acid and 4,5-O-dicaffeoylquinic acid in Chrysanthemi Indici Flos.