纤维素酶法提取土人参多糖的工艺研究

Research on the Extraction Process of Polysaccharide from Talinum Crassifolium Using Cellulase

摘要: 目的优化纤维素酶法提取土人参多糖。方法通过单因素实验和正交实验，研究pH值、酶用量、酶解温度和提取时间对土人参多糖提取效果的影响。结果纤维素酶法提取的优化工艺条件为：pH值为6，每克药材酶用量为400 U，酶解温度50 ℃，提取时间3 h。在此条件下，土人参多糖的提取率平均为21.16％，RSD为0.14％。结论纤维素酶法提取土人参多糖效果省时高效。

Abstract: OBJECTIVE To opmtimize the extraction technique for total polysaccharide from Talinum Crassifolium. METHODS The single factor experiment and the orthogonal design were conducted to investigate the influence of pH, enzyme dosage, extraction temperature and extraction time on yield of Polysaccharide from Talinum Crassifolium. RESULTS The optimum conditions of cellulose enzyme were as follows: pH 6.0, enzyme dosage 400 U·g^-1 enzymes drug, temperature 50 ℃, extraction time 3 h, and extraction yield was 21.16, RSD was 0.14%. CONCLUSION Extraction polysaeeharide from Talinum Crassifolium by cellulose enzyme is a simple, quick and efficient method．