Abstract: OBJECTIVE To study the optimal technological factors for extracting Arnebia euchroma polysaccharide with compound enzymes under ultrasonic．METHODS To develop an optimal procedure based on ultrasonic pretreatment followed by enzymatic hydrolysis for the extraction of polysaccharides from Arnebia euchroma, single factor and orthogonal design methods were used to deal with the effects of crucial ultrasonic pretreatment and hydrolysis parameters on polysaccharide yield. RESULTS The optimal values of ultrasonic power, pretreatment time and material/liquid ratio were determined to be 160 W, 40 min and 1∶12, respectively. The optimization of enzymatic hydrolysis was achieved through the combinatorial use of 2% cellulose, 2% pectinase and 2% neutral proteinase for the hydrolysis for 40 min at pH 5.5 and 55 ℃. CONCLUSION The combination of ultrasonic pretreatment and enzymatic hydrolysis may provide a good idea for the large-scale production of polysaccharides from Arnebia euchroma.