Abstract: OBJECTIVE To establish the analytical method for the fingerprint of Glycyrrhizae Radix et Rhizoma by HPCE-DAD and compare the fingerprints of Glycyrrhizae Radix et Rhizoma and its processed products. METHODS Based on the mode of high performance capillary electrophoresis, 40 mmol·L-1 sodium borate-10 mmol·L^-1 sodium dihydrogen phosphate- 10% methanol (pH 8.6) was used as buffer solution. The separation voltage was 20 kV and the detection wavelength was set at 254 nm. Glycyrrhizic acid was used as reference standard, the chromatographic fingerprint were determined. The data were analyzed by fuzzy cluster and fingerprint similarity evaluation software to compare the similarity of samples. RESULTS HPCE-DAD fingerprints with 10 common peaks of Glycyrrhizae Radix et Rhizoma were established preliminarily. It was discovered that a small number of samples differed from others. Regarding to the fingerprints of Glycyrrhizae Radix et Rhizoma and its processed products, there were obvious differences in the relative areas of common peaks. CONCLUSION The method is reliable, accurate and can be used for quality control of Glycyrrhizae Radix et Rhizoma.