Abstract: OBJECTIVE To establish a method for fast identification by TLC and quantitative determination by fluorescence TLCS for Qingwei Huanglian pills. METHODS Scutellariae Radix, Moutan Coxtex, Forsythiae Fructus, Gardeniae Fructus, Glycyrrhizae Radix et Rhizoma were identified by TLC, using the same sample solution in two TLC plate. The content of berberine hydrochloride in Qingwei Huanglian pills was determined by fluorescence TLCS. RESULTS The methodological study showed a good linear correlation in the range from 0.019 68 to 0.098 4 μg of the berberine hydrochloride. The average recovery of berberine hydrochloride (n=9) was 99.41%, and RSD was 1.34%. CONCLUSION The method is simple, accurate, repeatable and with good precision. The method can be used for quality control of Qingwei Huanglian pills. The detection speed was effectively improved for assay of traditional pills.