Abstract: OBJECTIVE To investigate if baicalin was able to cure antibiotic-resistant plasmid in Acinetobacter baumanii, and affect minimum inhibitory concentrations (MIC) of antibiotics to bacteria. METHODS The growth curve of A. baumanii treated with baicalin was detected. 16 strains of A. baumanii, gentamicin(GEN) and ciprofloxacin(CIP) resistance, were treated with baicalin for 20 hours. Before and after baicalin treatment, plasmids of A. baumanii were determined with alkaline lysis method, and MICs of GEN and CIP were measured by agar dilution method. RESULTS Baicalin inhibited the growth of A. baumanii. The plasmid curing rate was 37.5%(6/16) in 16 strains of A. baumanii with plasmids, after the treatment with baicalin (1 mg.mL^-1). In 6 strains of A. baumanii with plasmid cured by baicalin, for medium or low level of GEN-resistance strains, MICs of GEN all decreased to sensitive level; for low level of CIP-resistance strains, MICs of CIP all dropped to sensitive level, and for some medium-level of CIP-resistance strains, MICs of CIP also dropped, but still above the resistance level. CONCLUSION Baicalin can eliminate plasmids of A. baumanii with low level of antibiotic-resistance, and make these strains recover the susceptibility to GEN and CIP, which shows an adjuvant treatment method for antibiotic-resistant A. baumanii infections.