类风关巴布剂中雷公藤甲素的提取方法研究

Study on the Optimized Extraction Technology of Triptolide in Rhemeutoid-arthrosis Cataplasm

摘要: 目的研究并优化类风关巴布剂中雷公藤甲素的提取方法，并测定其含量。方法 HPLC测定类风关巴布剂中的雷公藤甲素含量，采用正交试验设计法考察甲醇浓度、甲醇体积、浸渍时间、提取时间对雷公藤甲素提取率的影响。结果最佳提取条件为用100 mL的60%甲醇，浸渍4 h，加热回流2 h提取效率最高。结论本方法为类风关巴布膏的质量控制提供了可靠的方法。

Abstract: OBJECTIVE To study and optimize the extraction method of triptolide in rhemeutoid-arthrosis cataplasm. METHODS With the quantitative determination of triptolide in rhemeutoid-arthrosis cataplasm by HPLC method, orthogonal test was used to evaluate the extraction, factors including the methanol concentration, methanol volume, soaking time, extraction time. RESULTS The optimal condition was using 100 mL of 60% methanol, with soaking time of 4 h and refluxing time of 2 h. CONCLUSION The optimized method is reliable for the quality control of cataplasm.