Abstract: OBJECTIVE To observe the effect of bovine lactoferrin(bLF) on the treatment of rotavirus-infected tree shrew. METHODS After establishment of animal model of tree threw with totavirus-infected, the animal model was treated with distinct doses of bLF. RESULTS The death rates of totavirus-infected tree shrew in bLF treatment group with high, middle and low doses were 10%, 20% and 60%.The mean days of death were 9.3, 8.9, 7.0 d. The rate of the prolonged survival time were 93.7%, 85.4%, 45.8%. Compared with infection-control group with no bLF treatment, the death rates and the mean days of death were reduced remarkably. CONCLUSION bLF can reduce the mortality rate of rotavirus infections of animals effectively and improve the life-prolonging rate, which can also reduce the rotavirus antigen titer.