Abstract: OBJECTIVE To detect the quantity of endotoxin in huangqi injection by the method of kinetic turbidimetric limulus test. METHODS The kinetic turbidimetric limulus test was applied to detect the quantity of endotoxin in several samples. The standard endotoxin working curve was set up and the interference test was studied to range the effective concentrations of sample. RESULTS Dilution ratio of Huangqi injection at 1∶5 show no inference in the test. The endotoxin recovery rate was more than 50% and less than 200%. The endotoxin level in samples could be quantified exactly, and the results were accorded with the results obtained by gel-clot technique assay. CONCLUSION The kinetic turbidimetric limulus test can be used to quantitate the endotoxin level in huangqi injection.