Abstract: OBJECTIVE To optimize the extraction technology of Rhodiola algida. METHODS Rhodiola algida was extracted by ultrasonic, refluxing and cold soaking extraction, respectively. The extraction rate, content of salidroside and p-tyrosol were chosen as the assessment index by using the orthogonal design. The effects of ethanol content, solvent volume, extracting time (hour) and extraction temperature were investigated. RESULTS The results indicated that the refluxing extraction was the best method. The optimized extraction process conditions were as follows: adding 8 fold of 50% ethanol, extracting for 1.0 h and 2 times at 80 ℃. CONCLUSION This study will provide a reference to the research of Rhodiola algida and industrial production.