Abstract:
OBJECTIVE To establish a method for the determination of the mixtures of seven fragrance ingredients, including geraniol, hydroxy citronellal, cinnamaldehyde, eugenol, amylcinnamic aldehyde, cinnamyl alcohol and isoeugenol,in patch paster which can be used for testing type IV allergy by capillary gas chromatography. METHODS Each patch was extracted with 1 mL ethanol for 30 minutes. The extraction liquid was injected into GC for separation with a polar PEG-20M capillary column. The external standard method was used for quantitative analysis. RESULTS The standard curves were linear on a certain concentration with correlation coefficients larger than 0.999 5 for the seven target fragrances. The relative standard deviations (
n=8) of precision and stability were less than 2%. The average recoveries for seven fragrances were between 95.48% and 107.52% with RSD(
n=3) in the range of 0.31%~2.00%. The relative standard deviations (
n=8) of uniformity were less than 5%. The quantitative limits for seven fragrances were 3.7, 4.3, 3.3, 3.5, 2.8, 2.3, 2.9 μg·mL
-1 respectively. CONCLUSION This method is simple, rapid, sensitive and accurate. It could meet the requirement for the quantitative analysis of fragrance ingredients in patch paster.