Abstract: OBJECTIVE To analyze the contents of total alkaloid in Aconitum leucostomum Worosch from different districts. METHODS The total alkaloids in different samples were isolated by Soxhlet extraction combined with different pH value solutions and extraction method. Ultraviolet spectrometer was used to analyze the contents of total alkaloid in all samples. RESULTS The contents of total alkaloid were 2.246, 4.682 and 3.597 mg·g^-1 in the roots of A. leucostomum Worosch from Xinyuan county, Gongliu county and Zhaosu county, respectively. The regression equation was Y=11.5X+0.121, r=0.999 6, and the concentration of aconitine within 6-36 μg·mL^-1 appeared the favorable linear relationship. CONCLUSION The ultraviolet-visible spectrophotometry is a simple and accurate method for determining the total alkaloid in A. leucostomum Worosch from different habitats. The results may establish foundations for further research on components in it.