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    青海产小叶黑柴胡中皂苷类成分的含量分析

    Content Analysis on Saponins in Bupleurum Smithii Wolff var. Parvifolium Shan et Y. Li

      摘要
    • 摘要: 目的 测定青海产小叶黑柴胡中总皂苷、柴胡皂苷a及柴胡皂苷d的含量，为其质量评价和开发利用提供参考。方法 采用分光光度法测定小叶黑柴胡中总皂苷的含量；采用高效液相色谱法测定小叶黑柴胡中柴胡皂苷a和柴胡皂苷d的含量。结果 小叶黑柴胡中总皂苷含量>2.77%，柴胡皂苷a和柴胡皂苷d的含量分别高于0.54%和0.14%，其中柴胡皂苷a和柴胡皂苷d的含量远高于中国药典中柴胡含量项下的标准。结论 本方法简便快速，结果准确、稳定，适用于小叶黑柴胡中皂苷类成分的含量测定。青海产小叶黑柴胡具有较高的药用价值。

       

      Abstract: OBJECTIVE To provide reference for exploitation and the quality evaluation of Bupleurum smithii Wolff var. parvifolium Shan et Y. Li in Qinghai province, and to determine the content of total saponins, saikoside a and saikoside d in it. METHODS The content of total saponins was determined by spectrophotometry. The content of saikoside a and saikoside d were determined by HPLC. RESULTS The content of total saponins, saikoside a and saikoside d in Bupleurum smithii Wolff var. parvifolium Shan et Y. Li were more than 2.77%, 0.54%, 0.14%, respectively. The content of saikoside a and saikoside d were higher than quality criteria in China Pharmacopeia. CONCLUSION The method is simple, rapid and accurate. The mothod is suitable for quality analysis of total saponins, saikoside a and saikoside d in Bupleurum smithii Wolff var. parvifolium Shan et Y. Li in Qinghai province which possess high medicinal value.

       

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