口服丁香酚在大鼠体内药动学研究

Study on Pharmacokinetics of Oral Eugenol in Rats

摘要: 目的建立大鼠血浆中丁香酚含量测定的高效液相色谱法(HPLC)。方法丁香酚大鼠灌胃给药后，应用HPLC测定其在大鼠血浆中的含量，色谱柱：Allsphere ODS-2 RP-18(4.6 mm×250 mm，5 μm)，流动相：甲醇-0.5%冰醋酸=63∶37 (pH=4.78)；检测波长：280 nm；柱温：30 ℃；流速：0.8 mL·min^-1。结果丁香酚经口服 14.25 mg·kg^-1的剂量后在体内药动学特征符合二室开放模型。结论所建方法简单、灵敏、准确可靠，可用于丁香酚的药动学研究。

Abstract: OBJECTIVE To establish an HPLC method for determination of eugenol in rat plasma. METHODS HPLC was applied to assay the eugenol concentration in rat plasma after oral administration. The separation was carried out on a Allsphere ODS-2 RP-18(4.6 mm×250 mm, 5 μm) column using the mobile phase: methanol-0.5% acetic acid (63∶37), pH=4.78. The detection wavelength was set at 280 nm with the column temperature 30 ℃. The flow rate was 0.8 mL·min^-1. RESULTS Eugenol pharmacokinetics fit the two-rooms open model after oral administration in rats with a 14.25 mg·kg^-1 dosage. CONCLUSION The method is simple, sensitive and accurate, and could be applied to the pharmacokinetic study of eugenol in rats.