Abstract: To establish a method to determine and study the content of b-elemene in Lantana camara Linn in Panxi area of Sichuan. METHODS The content of b-elemene was determined by HPLC with an Eclipse XDB-C₁₈ (4.6 mm×150 mm，5 mm) column, mobile phase EtOH-CAN-H₂O (70︰10︰20), flow rate 1.0 mL·min^-1, and UV detection wavelength 210 nm. The qualitative analysis of b-elemene was conducted by TLC and UV spectrum. RESUALTS A good linear relationship at the range of 0.007 5-0.120 0 mg·mL^-1(r=0.999 5) was obtained and the average recovery was 98.02%. b-elemene was tested out in leaves and flowers of Lantana camara Linn and not found in their roots, stems and fruits. The fresh leaves and naturally dried leaves had the highest content, which were higher notably than that of 60 ℃ dried leaves. The b-elemene contents in fresh leaves and flowers were 0.025% and 0.007%, respectively. CONCLUSION The mothod is suitable for b-elemene determination of Lantana camara Linn. The experiment can be used as references to find new resource of b-elemene and utilize Lantana camara Linn.