HPLC测定祛痰合剂中橙皮苷的含量

Determination of Hesperdin in Expectorant Mixture by HPLC

摘要: 目的建立高效液相色谱法测定祛痰合剂中橙皮苷的含量。方法采用Agilent Eclipse XDB-C₁₈柱(250 mm×4.6 mm，5 μm)，流动相为甲醇-冰醋酸-水(25∶5∶70)，流速1 mL·min^-1，检测波长283 nm，柱温30 ℃。结果橙皮苷线性范围是1.05~34.56 μg·mL^-1，线性方程为Y=10.346X+0.795 2，r=0.999 6；平均回收率为98.8%，RSD为1.2%(n=9) 。结论本法简便，准确和选择性好，可用于祛痰合剂的质量控制。

Abstract: OBJECTIVE An HPLC method was developed and validated for determining hesperdin in Expectorant mixture. METHODS Chromatographic separation was performed on an Agilent Eclipse XDB-C₁₈ column (250 mm×4.6 mm, 5 μm) with a mobile phase of methanol-glacial acetic acid-distilled water(25∶5∶70). The flow rate was 1.0 mL·min^-1 and the detecting wavelength was 283 nm. RESULTS The linear range of hesperdin was 1.05-34.56 μg·mL^-1. Linear equation was Y=10.346X+0.795 2(r=0.999 6). The average recovery (n=9) of hesperdin was 98.3% (RSD=1.7%). CONCLUSION The method is simple, accurate and selective and sensitive to quantify hesperdin in Expectorant mixture.