Abstract: OBJECTIVE To observe hepatoprotective effect and mechanism of TCCJEYS. METHODS The acute hepatic injury model was induced by D-GalN in order to observe hepatoprotective effect and mechanism of TCCJEYS. RESULTS Liver index(LI), the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and malondialdehyde (MDA) in liver tissue homogenate were significantly reduced by TCCJEYS on acute hepatic injury in mice. The levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in liver tissue homogenate were significantly increased. Meanwhile, the expression of B cell lymphoma/lewkmia-2 (Bcl-2) protein in liver tissue was improved, whereas the expression of factor associated suicide (Fas) protein in liver tissue was inhibited by TCCJEYS. HE dyeing also showed that TCCJEYS could improve pathological lesion. CONCLUSION TCCJEYS has protective effect on acute hepatic injury in mice induced by D-GalN.