Abstract: OBJECTIVE To establish a objective evaluation method on extraction process of Puyi granules. METHODS Orthogonal experiment was designed with the dynamic change of emodin, polysaccharide and caffeic acid as evaluation index to screen the best extraction process. RESULTS The best extraction conditions were as follows: Coix lacryma-jobi and Polygonum multiflorum in the prescription were smashed into coarse and mixed with 8 times of 80% ethanol to reflux for 2 times，one hours for each time. Then dregs were mixed Taraxacum mongolicum and Ganoderma lucidum in the prescription using 10 times of water to decoct 3 times，one hours for each time. CONCLUSION This method is accurate and reliable to evaluate the extraction process of Puyi for granules.