Abstract: OBJECTIVE To establish an HPLC fingerprint of Jiangzhuo Tongmai’s effective parts for controlling the quality of Jiangzhuo Tongmai’s effective parts. METHODS HPLC was used to create fingerprints with Sinochrom ODS-BP C₁₈ column (250 mm×4.6 mm, 5 μm) of Dalian Elite, the mobile phase was a mixture of acetontrile-0.1% phosphate water, a gradient eluting agent. RESULTS The fingerprint of Jiangzhuo Tongmai’s effective parts was established, and the detection wavelength was 203, 250 and 280 nm. CONCLUSION The quality of Jiangzhuo Tongmai’s effective parts can be controlled by the HPLC fingerprint. The method can be used for the quality control of Jiangzhuo Tongmai’s effective parts.