Abstract: OBJECTIVE To study the effect of the saponins of Rhizoma Paridis Yunnanensis Rhizoma from Paris polyphylla Smith var. yunnanensis(Franch.) Hand.-Mazz. on male mice procreation. METHODS The pennogenyl saponins(PHAC-A) and dioscins(PHAC-B) was extracted and purified from Rhizoma Paridis Yunnanensis. In the experiment of mice in vitro, the spermicidal effect on male mice of the PHACs in different concentration was measured. RESULTS The spermicidal effect in vitro on male mice of the PHACs was provided with excellent inhibition result in antifertility activities. Compared with gossypol acetic acid, PHACs showed a good inhibition result in spermicidal activities and survival rate, at the same time, PHAC-B had more inhibiting activities than PHAC-A, and had a direct proportion of the activities with their dosage. CONCLUSION Both of the PHACs showed the high inhibitory effect of the sperm activity and survival rate on mice procreation, and they were excellent activitie-compounds in antifertility ability. Compared with PHAC-A and PHAC-B, in Rhizoma Paridis Yunnanensis, the higher activity composition was the PHAC-B.