Abstract: OBJECTIVE To explore the optimal process conditions of pilot tests in multi-stage countercurrent extraction of resveratrol from Polygonum cuspidatum, and the measure of reducing solvent and alkali dosage. METHODS Multi-stage countercurrent extraction of resveratrol was studied. The extraction technology was discussed with the effects of the ratio of the CaO to the raw material, extraction team and the ratio of solid material to the extraction solvent. The four factors were selected in the orthogonal design, and the optimal extraction were processed by range analytic method and analysis of variance. Then compared this method with other unaided extraction process. RESULTS The ratio of the CaO to the raw material influenced the result significantly, the multi-stage countercurrent extraction is better than the unaided extraction process. CONCLUSION The optimal process conditions of pilot tests in multi-stage countercurrent extraction of resveratrol from Polygonum cuspidatum are as follows: the ratio of CaO to the solid material is 6∶100, the extraction team is four and the ratio of solid material to solvent is 1∶5. The process is more suitable to industrial production than other process in literature search.