HPLC-ELSD测定爱可福颗粒中的黄芪甲苷
Determination of Astragaloside Ⅳ in Aikefu Granules by HPLC-ELSD
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摘要: 目的 建立爱可福颗粒的定量质控方法。方法 采用HPLC-ELSD测定爱可福颗粒中黄芪甲苷的含量。结果 黄芪甲苷进样量在0.64~20.48 μg内呈良好的线性关系,平均回收率为97.78%,RSD=1.12%(n=6)。结论 该方法简便,重复性好,可用于控制爱可福颗粒的质量。Abstract: OBJECTIVE To establish a method for the determination of astragaloside Ⅳ in Aikefu granules by HPLC-ELSD. METHODS The content of astragaloside Ⅳ was determined by HPLC-ELSD. RESULTS A good linearity was observed for astragaloside Ⅳ in the range of 0.64-20.48 μg. The average recovery of astragaloside Ⅳ was 97.78% with RSD=1.12%(n=6). CONCLUSION The method is simple, accurate and reiable. It can be used for the quality control of granules.