Abstract: OBJECTIVE To establish the UPLC fingerprint analysis method for the quality evaluation of Rhizoma Atractylodis Maerocephala. METHODS UPLC fingerprint analysis method was applied for quality evaluation. ACQUITY UPLC BEH C₁₈ column was used, with the mixture of acetonitrile and water as mobile phase in a gradient mode. The flow rate was 0.1 mL·min^-1. The wavelength of measurement was 242 nm. Thirty-two batches of Rhizoma Atractylodis Maerocephalae were determined. RESULTS Most of the peaks were separated effectively, and thirty-two mutual peaks were selected from chromatography. The RSD of relative retention time of these mutual peaks was less than 1.0%, and the similarity of all samples was over 90%. CONCLUSION The method is provided with high resolving power and sensitiveness, can shorten the analyze time effectively, will be used to identify and evaluate the quality of Rhizoma Atractylodis Maerocephalae conveniently.