Abstract: OBJECTIVE To investigate and compare the effect of different extracts of Tussilago farara L. on the contraction of isolated ileum of guinea pig. METHODS By the method of petroleum, acetoacetate and supercritical CO2 , the Tussilago Farara L. was extracted Isolated ileum of guinea pig was put in K-H solution. The contraction extent of isolated ileum was observed. Inhibition ratio of the contraction extent was calculated. RESULTS Different extracts of Tussilago Farara L. decreased the contraction extent of isolated ileum of guinea pig cased by histamine. CONCLUSION The extracts of Tussilago Farara L. significantly inhibited the contraction of isolated ileum of guinea pig .