LC-MS/MS快速测定生物检材中乌头碱的含量

A Rapid Method for Determination of Aconitine in Biology Samples by LC-MS/MS

摘要: 目的建立快速测定生物检材中乌头碱的LC-MS/MS方法。方法生物检材经乙醚-二氯甲烷(3∶2)提取后，用Venusil Mp-C₁₈柱进行分离，采用ESI多反应监测(MRM)扫描方式。结果测定的线性范围为3~5 000 ng·mL^-1，RSD<1.8%，日间和日内精密度分别为2.5%和4.6%，提取回收率为85.61%~89.04%。结论该方法简便、快捷，适用于生物检材中乌头碱的快速测定。

Abstract: OBJECTIVE To develop a rapid method for determination of aconitine in biology samples by LC-MS/MS. METHODS Aconitine and the internal standard tramadol were extracted from biology samples using diethyl ether -dichloromethane (3∶2), and separated on a Venusil Mp-C₁₈ column. Detection was carried out by multiple reaction monitoring (MRM) on a Q-trapTM LC-MS/MS system with an ESI interface in the positive ion mode. RESULTS The linear arrang was 3-5 000 ng·mL^-1. The accuracy was 1.8% and the intra- and inter-day precision were 2.5% and 4.6%, respectively. The recovery was between 85.61% and 89.04%. CONCLUSION This method is simple and rapid, which can be used for the determination of aconitine in biology samples by LC-MS/MS.