HPLC测定利胆合剂中白藜芦醇含量

Determination of Resveratrol in Longlianlidan Mixture by HPLC

摘要: 目的建立HPLC测定利胆合剂中白藜芦醇含量的方法。方法采用HYPERSIL ODS C₁₈色谱柱(250 mm×4.6 mm，10 µm)；乙腈-水(25∶75)为流动相；流速为1 mL·min^–1；检测波长为305 nm。结果白藜芦醇在8.2~164 µg·mL^–1内呈现良好的线性关系，r=0.999 7，重现性良好，平均回收率为87.02%，88.31%，90.67%。结论本法检测快速，定量准确，可用于利胆合剂中白藜芦醇的含量测定。

Abstract: OBJECTIVE To establish an HPLC method for determination of resveratrol in Longlianlidan mixture. METHODS The determination of resveratrol in Longlianlidan mixture by HPLC was performed with HYPERSIL ODS C₁₈(250 mm×4.6 mm, 10 µm) and the mobile phase of acetonitrile-water(25∶75) at a flow rate of 1.0 mL·min^–1, and the detection wavelength was conducted at 305 nm and at room temperature. RESULTS The calibration curve was linear within the range of 8.2-164 µg·mL^–1 and the average recovery of resveratrol was 84.56%, 88.31%, 90.67%, respectively. CONCLUSION The HPLC method is precise, quick and suitable for determination of resveratrol in Longlianlidan mixtrue.