Abstract: OBJECTIVE To establish an HPLC method to determine the content of clindamycin in muscle of rabbits, and to study its characteristics of distribution in muscle tissue of rabbits which were stopped bleeding by tourniquet. METHODS The mobile phase consisted of a mixture of acetonitrile-tetrahydrofuran-phosphoric acid(30∶1∶70, pH=5) with a flow rate of 0.8 mL·min^-1. Tinidazole was used as an internal standard, the analytical column was packed with 5 μm C18 at 40 ℃, and the detection wavelength was 205 nm. Experiment groups were stopped bleeding for 30, 60, 90 min after drug administration at 30, 60, 90, 120 min, respectively. Then, clindamycin in muscle of exeriment and control groups were determined by HPLC. RESULTS The calibration curve of clindamycin was linear in the range of 10.58-264.5 μg·mL^-1. The RSD of intra and inter-day were smaller than 2.58% and 4.20%, respectively. The concentration of clindamycin in exeriment group compared with that in control group was significant different(P<0.01). In the same stopped bleeding occasion groups, multiple comparison among the different time length of stopping bleeding groups was not significant different (P>0.05), multiple comparison among the different occasion of stopping bleeding groups (30, 60, 90, 120 min) was significant different (P<0.01). The concentration of clindamycin was 39.30 μg·g^-1 in the group stopped bleeding after drug administration at 60 min, it is the highest content in exeriment groups. CONCLUSION This method is accurate, reliable, simple and reproducible. The difference of concentrations of clindamycin in experiment groups compared with that in control groups is significant. Preventing infection in perioperative period, the occasion of stopping bleeding is the principal factor to affect the distribution of clindamycin in muscle tissue, stopping bleeding after drug administration at 60 min can make medicine distribute to muscle tissue completely, and enhance the efficiency of infection prevention.