Abstract: OBJECTIVE To study the inhibitory effects of traditional Chinese medicine Yiganzhuanyinsan (YGZYS) on hepatitis B surface antigen (DHBsAg) and hepatitis B e-antigen (DHBeAg). METHODS One-day old Guangxi brown spotted ducks infected with hepatitis B virus (DHBV) were used as the DHBV infected animal model. Positive ducks were detected by PCR at one week after the infection of DHBV, and were randomly divided into five groups: the high dose group, middle dose group and low dose group of Yiganzhuanyinsan(YGZYS), model group and positive control group, with 10 ducks in each group. YGZYS was given ig. for 14 days. Serum samples were collected on the 0, 7 th, 14 th day respectively after the treatment with drugs and the 3rd day after drug withdrawal, and the contents of DHBsAg and DHBeAg in serum were measured by ELISA. RESULTS The serum DHBsAg and DHBeAg contents in high dose and middle dose groups of YGZYS were decreased significantly (P<0.01 or P<0.05), and did not rebound on the 3rd day after drug withdrawal, and its inhibitory effects on DHBsAg and DHBeAg were an obvious dose-and time-dependent manners. CONCLUSION YGZYS can effectively inhibit the expressions of DHBsAg and DHBeAg.