Abstract: OBJECTIVE Electrochemical behaviors, electrochemical kinetics and electrochemical quantitative determination of tinidazole (TNZ) at glassy carbon electrode (GCE) and room temperature ionic liquid-multi-wall carbon nanotubes modified glassy carbon electrode (RTIL-MWCNTs/GCE) were investigated. METHODS Cyclic voltammetry (CV), chronocoulometry (CC), chronoamperometry (CA), square wave voltammetry (SWV), amperometric I-t curve and alternating current-inpedance (AC-inpedance). RESULTS The reduction peak current of TNZ increased at RTIL-MWCNTs/GCE in comparison with that at the bare GCE and MWCNTs/GCE. At RTIL-MWCNTs/GCE, the reduction peak current was proportional to TNZ concentration over the range of 5.0×10^-5-1.0×10^-2 mol·L^-1. The detection limit was 2.4×10^-6 mol·L^-1. The relative standard deviation was between 0.64%-1.65% and the recoverage was in the range of 98.7%-100.2%. CONCLUSION RTIL- MWCNTs/GCE could catalyze TNZ electrochemical reduction very well, and it was a diffusion-limited electrochemical process. The proposed method could be applied in the electrochemical determination of TNZ content in injection samples with the satisfied results.