HPLC测定盐酸度洛西汀的含量

HPLC Determination of Duloxetine Hydrochloride

摘要: 目的建立高效液相色谱法测定盐酸度洛西汀含量的方法。方法采用Kromasil C₈柱(250 mm×4.6 mm，5 μm)，流动相为乙腈-0.01 mol·L^-1磷酸氢二钠溶液(pH 6.0)(45∶55)，流速为1.0 mL·min^-1，检测波长为290 nm。结果盐酸度洛西汀的线性范围为8.104~12.156 μg·mL^-1(r=0.999 9)。盐酸度洛西汀平均含量为99.72%，RSD为0.66%。结论该方法简便、准确、专属，可用于盐酸度洛西汀的含量测定。

Abstract: OBJECTIVE To establish an HPLC method for the determination of duloxetine hydrochloride. METHODS The Kromasil C₈(250 mm×4.6 mm, 5 μm) was used. The mobile phase was acetonitrible-0.02 mol·L^-1 disodium hydrogen phosphate solution (pH 6.0) (45∶55). The flow rate was 1.0 mL·min^-1. The detection wavelength was 290 nm. RESULTS The calibration curve was linear in the range of 8.104-12.156 μg·mL^-1 (r=0.999 9). The average content of duloxetine hydrochloride was 99.72% and RSD was 0.66%. CONCLUSION The mothod is sample, accurate, specify and can be used for the determination of duloxetine hydrochloride.