Abstract: OBJECTVE To evaluate the clinical application of vancomycin use in 33 cases in our hospital. METHODS The 33 cases of patients who took vancomycin while their drug concentration in blood was monitored between Jan 2008 and Jan 2009 were reviewed and analyzed statistically. RESULTS In the 33 cases, the efficiency of clinical treatment was 57.58％, and the drug utilization index(DUI) was 0.609. CONCLUSION The use of vancomycin in our hospital was rational in general. The vancomycin concentration in blood should be monitored meanwhile because of the narrow therapeutic window of vancomycin.