正交试验法优选墨旱莲多糖的水提工艺研究

Optimal Water-Extraction of Polysaccharide from Herba Ecliptae by Orthogonal Design

摘要: 目的研究墨旱莲多糖的最佳水提工艺。方法以墨旱莲中多糖的含量和提取率为指标，采用正交试验筛选墨旱莲多糖的最佳提取工艺。结果最佳工艺参数为料水比1∶10，提取3次，每次3.5 h，提取温度100 ℃。在此条件下，测得墨旱莲多糖提取率为11.39%，多糖含量为18.43%。结论本实验优选出的工艺稳定、经济、可行。

Abstract: OBJECTIVE To optimize the water-extraction of polysaccharide from Herba Ecliptae. METHODS With the extraction rate and contents of polysaccharide as the index, orthogonal test was used to evaluate the extraction. RESULTS The optimal condition was added 10 times water for 3 times and 3.5 hours for each time at the temperature of 100 ℃. In this condition, the extraction percent of polysaccharide from Herba Ecliptae was 11.39% and the content was 18.43%. CONCLUSION The optimized extraction is stable, economical and feasible.