柴白安神合剂的质量控制研究

Study on Quality Control of Chaibaiansheng Mixture

摘要: 目的建立柴白安神合剂的质量标准，有效控制柴白安神合剂的质量。方法采用薄层色谱法(TLC)对柴胡、百合、白芍、五味子、川芎进行定性鉴别，高效液相色谱法(HPLC)对黄芩苷进行含量测定。结果 TLC可定性鉴别柴胡、百合、白芍、五味子、川芎5味药材，且Rf值合理，重复性好；HPLC中黄芩苷在0.103 2~1.032 μg内线性关系良好(r=0.999 8)，平均回收率为99.5%，RSD为1.4%(n=6)。结论该质量控制方法准确，简单、专属性强，可有效控制柴白安神合剂的质量。

Abstract: OBJECTIVE To establish the quality standards for quality control of Chaibaiansheng mixture. METHODS TLC was adopted to identify Radix Bupleurum, Bulbus Lily, Radix Paeoniae Alba, Fructus Schisandrae Chinensis and Rhizoma Chuanxiong, and a HPLC method was used to test the content of Baicalin. RESULTS TLC can identify these extraction: Radix Bupleuri, Bulbus Lilii, Radix Paeoniae Alba, Fructus Schisandrae Chinensis and Rhizoma Chuanxiong, and the Rf is reasonable and repeatable; Baicalin has good linearity relationship by HPLC in 0.103 2-1.032 μg(r=0.999 8), the average recovery is 99.7%, RSD is 1.5%(n=6). CONCLUSION This method of qualitative control is precise, easy and exclusive, and has good effect on quality control of Chaibaiansheng mixture.