Abstract: OBJECTIVE To investigate the effect of Chinese medicine Kanglaite injection on expression of cyclin modulin P16, P63 in HL-60/ADR cells. METHODS HL-60/ADR cells during logarithmic growth phase were treated with Kanglaite injection, growth curve was based on cell counting; the inhibitory effect of adriamycin(ADR) on Kanglaite injection-treated HL-60/ADR cells was tested by MTT assay; the concentration of intracellular adriamycin and the expression of P16, P63 were detected by flow cytometry. RESULTS In each group, live cell count reached a peak on the 6th day, compared with control group, the Kanglaite injection-treated cell count was lower with statistical significance(P<0.05). Treated HL-60/ADR cells with 1 mg·mL^-1 Kamglaite for 96 h, the inhibitory effect of adriamycin on the cells were greater than that of control groups(P<0.05). After treatment with different concentration of Kanglaite injection, the intrancellular ADR fiourescence of 1.5 μg·mL^-1 ADR-treated for 6 h were statistical higher than that of control group(P<0.05). Treated HL-60/ADR cells with 1 mg·mL^-1 Kamglaite for 48 h, compared with the control group, the expression of P16, P63 were all significantly increased(P<0.05). CONCLUSION HL-60/ADR cells exposed to Kanglaite injection resulted in dose-depentent inhibitory, Kanglaite injection could increase the sensitivity of HL-60/ADR cells to ADR. Kanglaite injection could increase the expression level of P16, P63 in HL-60/ADR cells, that maybe involved in the anti-tumor mechanics of Kanglaite injection.