Abstract: OBJECTIVE To study the intestinal absorption characteristics of forsythin in rats. METHODS Everted intestinal sac model was used and the concentration of forsythin was determined by HPLC. RESULTS There are no significant differences (P>0.05) between ka of forsythin at three concentrations (10, 20, and 40 μg·mL^-1) of forsythin in perfusate; quantity of forsythin absorbed at small intestine in rats is ileum>jejunum>duodenum; verapamil hydrochloride and Tween-80 have no influence on absorption of forsythin. CONCLUSION The absorption of forsythin shows the first rate absorption characterize in the rat intestine, it is passive diffusion process, forsythin is not the substrate of P-gp.