Abstract: OBJECTIVE To establish HPLC methods for determining salicylic acid and terbinafine hydrochloride in rabbits plasma, and to investigate the pharmacokinetics of salicylic acid and terbinafinie hydrochloride in Kemeishu liniment after percutaneous absorption in rabbits. METHODS 12 rabbits with their hair on the back removed were randomized divided into large single dose group, medium single dose group and low single dose group. Plasma samples were collected and quantitated by HPLC. The pharmacokinetic parameters were calculated using PKS1.0 software. RESULTS Terbinafine hydrochloride were not determined in rabibits plasma.The main pharmacokinetics parameter of salicylic acid in low, medium and large single dose group were as follows: Tmax (2.73±0.30), (3.48±0.16), (5.86±1.72)h, Cmax (25.24±9.21), (25.32±11.05), (37.47±4.31)µg·mL-1, T1/2 (5.74±3.29), (4.60±1.99), (13.28±3.84)h. The Tmax, Cmax, T1/2 in large single dose group were higher than that in medium and low single dose group (P<0.05). CONCLUSION The established methods are sensitive, fast and accurate, which were suitable for the determination of salicylic acid and terbinafine hydrochloride in plasma . Kemeishu liniment is safety spreding on skin, terbinafine hydrochloride seldomly can enter in the blood, salicylic acid partially enter in the blood, its pharmacokinetics fitted one-compartment model.