Abstract: OBJECTIVE A field experiment was conducted to investigate the different effects of various plant growth regulators on diosgenin contents and rhizome yields of Dioscorea zingiberensis. METHODS Experiments were conducted with one factor design , and data were analyzed statistically on SPSS 17.0. RESULTS Compared with the control, the rhizome yields were significantly increased by applying with 2, 4-D, NAA, paclobutrazol and uniconazole, but the contents of diosgenin was not obviously influenced by them. The degree of effect on diosgenin content is ranged with 2, 4-D, Pengdasu, NAA, uniconazole, paclobutrazol, while the outputs of dioscorea rhizome is 2, 4-D,NAA, paclobutrazol , uniconazole, Pengdasu. CONCLUSION 2,4-D can increase the diosgenin contents and rhizome yields of Dioscorea zingiberensis