Abstract: OBJECTIVE To investigate the chemical constituents of Salacia amplifolia. METHODS The chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography, their structures were elucidated by chemical and spectral methods. RESULTS Nine compounds were isolated and their structures was determined as 2- hydroxyfriedelan-3-one (1), friedelin (2), lup-20 (29)-en-3, 21-dione (3), D-friedoolean-14-en-3-one (4), 3-(3″, 4″-dihydroxy-trans- cinnamoyloxy)-D-friedoolean-14-en-28-oic acid (5), 3, 22-dioxo-29-normoretane (6), Lupeol (7), β-Sitosterol (8), β-Daucosterol (9). CONCLUSION Compounds 1-9 are isolated for the first time from the plant.