Abstract: OBJECTIVE To study the separatation and purification for geniposide and gardenia yellow from gardenia simultaneously with macroporous resin. METHODS Comparing the the static adsorption capacity of gardenia yellow was used to investigate different macroporous resin, and get the optimum purified process with the best macroporous resin. RESULTS The best macroporous resin: HPD-100 macroporous resin; and the purify process: the extracted solution(absorbency at 440nm was about 60) was passed through HPD-100 resin chromatographic column with flow rate of 1-3 mL·min-1, after which，eluted it with distilled water (8-10 times volum), 25% ethanol (double volum) and 70% ethanol to be almost colorless. CONCLUSION The percent of yield of geniposide was 70%, and that of gardenia yellow (color price between 100 to 500, the OD less than 0.8 or even 0.3) was 80% (equivalent to the content of medica gardenia), all proves that the technique condition is reasonable.