Abstract: OBJECTIVE To review the applications of mitomycin C in ophthalmology. METHODS On the base of the related literatures at home and abroad printed recently, applications and complications associated with mitomycin C were reviewed in pterygium surgery, glaucoma surgery, corneal refractive surgery, et al. RESULTS AND CONCLUSION Mitomycin C has been shown to be effective in ophthalmology, but significant complications have also been reported. Route of administration, concentration and duration of exposure to mitomycin C should be thought about, recurrence and complications would be reduced.