Abstract: OBJECTIVE To investigate the protective effects of Shu Xue Ning inject liquid on myocardial ischemia. METHODS The protective effect of Shu Xue Ning inject liquid on myocardial ischemia was investigated in myocardial ischemia rats induced by isoproterenol and hypophysin, myocardial ischemia dogs caused by coronary artery ligation and perfusion of isolated rat hearts. RESULTS Shu Xue Ning inject liquid significantly inhibited myocardial ischemia of rats induced by isoproterenol and hypophysin, significantly decreased the range of canis familiaris myocardial ischemia caused by coronary artery ligation, the electrical potential of epicardium revealed that the extend of ischemic decreased, increased the flow of aeteria coronaria. CONCLUSION These results show that Shu Xue Ning inject liquid can significantly dilate the blood vessels, increase the flow of aeteria coronaria, inhibit the range and the degree of myocardial ischemia induced by coronary artery ligation.