HPLC测定骨骼风痛胶囊中薯蓣皂苷元的含量

Determination of Diosgenin in Gugefengtong Capsule by RP-HPLC

摘要: 目的建立骨骼风痛胶囊中薯蓣皂苷元的含量测定方法。方法采用HPLC，色谱柱为Hypersil ODS2(4.6 mm×250 mm，5 μm)；流动相为甲醇-水(93∶7)；检测波长203 nm。结果薯蓣皂苷元在0.772 8～3.864 0 μg内与峰面积呈良好的线性关系，回归方程为Y=443 254X＋18 856，r=0.999 1；平均回收率为97.87%，RSD为1.71%。结论本法可用于骨骼风痛胶囊的质量控制。

Abstract: OBJECTIVE To establish an HPLC method for the determination of diosgenin in Gugefengtong capsule. METHODS diosgenin was separated on Hypersil ODS2 (4.6 mm 250 mm, 5 μm) column and detected at 203 nm, with methanol-water (93∶7) as mobile phase. RESULTS There was good linearity from 0.772 8 to 3.864 0 μg (Y= 443 254X＋18 856, r=0.999 1) in separation of diosgenin. The average recovery was 97.87% with RSD of 1.71%. CONCLUSION This method provides the reference to establish quality standard for Gugefengtong capsule.