Abstract: OBJECTIVE To introduce the Application of Aqueous two phase extraction in drug extraction and separation in the recent years. METHODS Summarize and analyze the literatures and data published in the recent years, review the basic principle of Aqueous two phase extraction technology and features, illustrate the technique application and development in protein, enzyme, amino acids drugs, abstraction and segregation of traditional Chinese medicine system and antibiotic. RESULTS The extensive application and development of ATPE have been conducted in recent years. CONCLUSION Application of ATPE to the drug extraction and separation has a bright future,and will impulse the development of pharmaceutical industry.