Abstract: OBJECTIVE To evaluate the sensitizations by intravenous of Houttuynia Cordata injections and polysorbate 80 injection. METHODS Active systemic anaphylaxis(ASA) test was carried out, by intravenous of different injections to Beagle dogs to immunize, and inject double doses of injections to stimulate in the 9 days after the last sensitized. Observe the anaphylaxis situation of Beagle dogs and measure the concentrationns of histamine, IgE, IgM, IgG in plasma with ELISA. RESULTS Polysorbate 80 injection and Houttuynia Cordata injections which include polysorbate 80 are positive on Beagle dogs after by intravenous, it's obviously behavior disorder and histamine elevation, but IgE is irregularly changed , IgM and IgG are not obvious variation. CONCLUSION Polysorbate 80 is associated with ADRS of Houttuynia Cordata Injection which leaded to severously allergic reactions. It is suggestion that anaphylactic reaction and anaphylactoid reaction tests of Beagle dogs should be carried out in the sensitization test of Chinese traditional medicine injections, behavior disorder and histamine in plasma are important assessment index, IgE is assisted one.